摘要 : The discovery of genome editing technology based on the bacterially derived CRISPR system (clustered regularly interspaced short palindromic repeats) has led to tremendous progress in biotechnology and gene therapy. The CRISPR/Cas... 展开 The discovery of genome editing technology based on the bacterially derived CRISPR system (clustered regularly interspaced short palindromic repeats) has led to tremendous progress in biotechnology and gene therapy. The CRISPR/Cas9 system has many advantages over other genome editing technologies, including multiplexing, high accuracy, cost-effectiveness, and simplicity. However, the safe and effective delivery of the genome editing system's components into the target cells, is one of the major challenges in genome editing. This has a direct effect on the success and therapeutic applications of this system. A variety of different delivery methods including physical delivery methods, viral and non-viral vectors are used to deliver CRISPR/Cas9 system com-ponents. However, employing physical delivery techniques and viral vectors has limitations, including cellular damage, immune system response induction, low specificity, need for expertise and expensive equipment, etc. Recently, the development and use of nanoparticle-based delivery systems for efficient delivery of the CRISPR/ Cas9 system has attracted significant attention. The application of nanoparticles for the delivery of CRISPR/Cas9 system components has been highlighted by benefits including simple synthesis, cost -effectiveness, size tunability, high loading capacity, high efficiency, non-mutagenicity, non-immunogenicity, etc. Here, we will review developments in the delivery of CRISPR/Cas9 system components employing lipid-based nanoparticles and highlight forthcoming challenges. 收起