摘要 : ? 2023 Elsevier B.V.The dermocistidiosys is a new fish disease in the Brazilian aquaculture. This is the second report occurred in Pangasionodon hypophthalmus in Brazil, but therapeutic alternatives to controlling it still are mis... 展开 ? 2023 Elsevier B.V.The dermocistidiosys is a new fish disease in the Brazilian aquaculture. This is the second report occurred in Pangasionodon hypophthalmus in Brazil, but therapeutic alternatives to controlling it still are missing. The objective of this study was to characterize the Dermocystidium spp. infection in P. hypophthalmus fingerling and to evaluate different alternatives for controlling it. A total of 170 fish naturally infected with Dermocystidium spp. were used in this study. The fish were anesthetized and then submitted to blood and parasitological analysis. The glucose, total of erythrocytes, hematocrit percentage, hemoglobin concentration, hematimetric indexes, thrombocytes and differential of leukocytes were evaluated. In vitro tests were performed exposing Dermocystidium spp. cysts at to different therapeutic products: copper nanoparticles (CuNP), zinc sulphate (ZnSO4), copper sulphate (CuSO4), plant aqueous extract of Costus spicatus and Schinus terebinthifolia. The plasmodium viability (live/dead) were evaluated by fluorescent probes. Afterwards, an in vivo assay was carried out using the same therapeutic products plus one treatment represented by elevating temperature (32o C) during 96 h, evaluating the clinical signs and mortality. After 96 h, the surviving fish were monitored for more 60 days. The infected fish before experiments, presented discolored skin areas and a worm-like elongated cysts (plasmodium) within vesicles randomly distributed through the body, showing mean intensity of 80.70 ± 23.46 cysts/infected fish and the neutrophil was the most abundant cell in the blood. All therapeutic products caused mortality of spores within cysts in vitro assay. However, for in vivo test, despite the efficacy in vitro, fish died after exposure to treatments, except to elevating temperature. The fish into the controlled temperature strategy demonstrated 100% of survival at the end of 96 h and absence of clinical signs after 60 days. 收起