摘要 : We developed eight PCR-primer pairs of polymorphic microsatellite loci for the tungara frog Physalaemus pustulosus. Genomic libraries were enriched for one of four microsatellite repeat sequences (CA_(n') GA_(n') ATG_n and TAGA_n)... 展开 We developed eight PCR-primer pairs of polymorphic microsatellite loci for the tungara frog Physalaemus pustulosus. Genomic libraries were enriched for one of four microsatellite repeat sequences (CA_(n') GA_(n') ATG_n and TAGA_n). Following characterization of microsatellite loci by sequencing, primers were designed and PCR conditions optimized. Microsatellite, PCR-amplification was tested in 37 frogs from 8 populations in Costa Rica and Panama. Primer sequences, PCR conditions, allelelic diversities and observed as well as expected heterozygosities in the screened populations are described. 收起