摘要 : In this study, the effects of the addition of L-carnitine in in vitro maturation (IVM) medium for bovine oocytes on theirnuclear maturation and cryopreservation were investigated; they were matured in IVM medium supplemented with ... 展开 In this study, the effects of the addition of L-carnitine in in vitro maturation (IVM) medium for bovine oocytes on theirnuclear maturation and cryopreservation were investigated; they were matured in IVM medium supplemented with 0.0,0.3, 0.6 and 1.2 mg/mL of L-carnitine (control, 0.3, 0.6 and 1.2 groups, respectively) and some of them were vitrified byCryotop. Moreover, the effects of L-carnitine during in vitro fertilization (IVF) and in vitro culture (IVC) on the developmentalpotential and quality of IVF embryos were also examined. A significantly higher maturation rate of oocytes was obtainedfor 0.3 and 0.6 mg/mL groups compared with the control (P < 0.05). The blastocyst formation rate in the 0.6 group wassignificantly improved, whereas the rate in the 1.2 group was significantly decreased when compared with the controlgroup (P < 0.05). No significant difference was found in embryo development between the control and the L-carnitinegroup after oocyte vitrification. Supplementation of IVF and IVC media with L-carnitine had no effect on development tothe blastocyst stage of IVM oocytes treated with 0.6 mg/mL L-carnitine. In conclusion, the supplementation of L-carnitineduring IVM of bovine oocytes improved their nuclear maturation and subsequent embryo development after IVF, but whenthey were vitrified the improving effects were neutralized. 收起