摘要 : Optimization of the polyhydroxybutyrate (PHB) production by a pigmented Micrococcus sp. was done by using glucose, sucrose, lactose and fructose as carbon sources, initial pH between 6.0 and 8.0 fermentation temperature of 30 and ... 展开 Optimization of the polyhydroxybutyrate (PHB) production by a pigmented Micrococcus sp. was done by using glucose, sucrose, lactose and fructose as carbon sources, initial pH between 6.0 and 8.0 fermentation temperature of 30 and 37 degrees C, inoculum of 5, 10 and 15% v/v and by submerged/aerobic fermentation. Maximum production of polyhydroxyalkanoates (PHA) was at 37 degrees C in sucrosc containing pH 6.0 under aerobic conditions. Overall, the PHB production ranged between 35 and 40% of the dry cell weight. In GC analysis, PHA and PHB produced the same peak. The UV-visible spectrophotometer and HPLC analysis of the pigment indicated presence of beta-carotenoids with absorption maxima at 454 and 348 nm. 收起