摘要 : Naturally, citrus blackfly mycosis nymphs were observed in citrus orchard in the vicinity of Vidarbha region of Maharashtra and the fungus colony recovered on SDA media using tissue isolation technique. The characterization of the... 展开 Naturally, citrus blackfly mycosis nymphs were observed in citrus orchard in the vicinity of Vidarbha region of Maharashtra and the fungus colony recovered on SDA media using tissue isolation technique. The characterization of the morphology involved mycelium, conidia, colony appearance and stroma types; the isolated fungus resulting from molecular recognition was Aschersonia aleyrodis. In vitro investigations were conducted to evaluate the pathogenesis of Aschersonia aleyrodis PDKV isolate against Aleurocanthus woglumi using a topical application method. Early infection of blackfly-by fungus was detected as white fringes of hyphae extending from the marginal area of the nymphal body. At the end of the infection, mat-like pustules were observed to cover the entire nymphal body. At first, colonies were white and became yellowish to orange in colour as stroma form. The effect of eleven chemical fungicides and seven insecticides commonly used in citrus crop was evaluated at half and recommended doses in vitro on the mycelial growth of A. aleyrodis. Maximum radial mycelial growth was achieved in mancozeb i.e. 4.3 cm and 1.8 cm at half and recommended doses, while carbendzaim, fosetyl Al, carbendazim + mancozeb (combi product), and tebuconozol + trifloxystrobin (combi product) completely inhibited the growth of A. aleyrodis at concentrations tested. Among the insecticides, dimethoate notably supported the maximum growth (5.2 cm) of A. aleyrodis over the rest of the insecticides at the concentrations tested. Present work shows that the most suitable insecticides and fungicides in conjunction with A. aleyrodis are to be used in Integrated Pest Management Programs. 收起