摘要 : We describe the isolation, biochemical characterization, phylogenetic analysis, and pathogenecity of a novel entomopathogenic bacterium <i>Brevibacterium frigoritolerans</i> to first instar larvae of <i>Anomala dimidiata</i> and <... 展开 We describe the isolation, biochemical characterization, phylogenetic analysis, and pathogenecity of a novel entomopathogenic bacterium <i>Brevibacterium frigoritolerans</i> to first instar larvae of <i>Anomala dimidiata</i> and <i>Holotrichia longipennis</i>. The almost full length 16S rRNA sequence of the bacterium has 99% identity with the type strain of <i>B. frigoritolerans</i>, while phylogenetic analysis revealed that the isolate formed a tightly linked branch with the type strain of <i>B. frigoritolerans</i>. Under <i>in vitro</i> bioassay conditions, the isolate infected and caused 89+or-5.4 and 74+or-7.7% mortality, in first instar larvae of <i>A. dimidiata</i> and <i>H. longipennis</i>, respectively. The infected larvae exhibited bacteremia like symptoms and mortality occurred between the second and fifth weeks after inoculation. This is an early report on the entomopathogenic potential of the hitherto lesser-known bacterium <i>Brevibacterium frigoritolerans</i>. 收起