摘要
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Three genes encoding proteins showing sequence similarity and features typical of insect APNs were characterized in <i>C. tremulae</i> and designed as <i>CtAPN1</i>, <i>CtAPN2</i> and <i>CtAPN3</i>. Expression analysis of the thre...
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Three genes encoding proteins showing sequence similarity and features typical of insect APNs were characterized in <i>C. tremulae</i> and designed as <i>CtAPN1</i>, <i>CtAPN2</i> and <i>CtAPN3</i>. Expression analysis of the three <i>C. tremulae</i> APN genes showed that <i>CtAPN2</i> transcript is more abundant in the fat body, whereas both <i>CtAPN1</i> and <i>CtAPN3</i> are specifically expressed in the midgut. Despite a similar genomic organization, lepidopteran and coleopteran APNs are phylogenetically distant, suggesting that APN gene duplication events occurred after these two insect orders split. Sequence and expression comparisons of <i>CtAPN1</i>, <i>CtAPN2</i> and <i>CtAPN3</i> cDNAs in a <i>C. tremulae Bacillus thuringiensis</i> (Bt)-susceptible and in a Bt-resistant strain did not show any polymorphism at the amino acid level or difference at the transcription level.Digital Object Identifier http://dx.doi.org/10.1111/j.1365-2583.2010.01067.x
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