摘要 : Gundelia tournefortii is a perennial spiny herb, and a neglected plant, found in the Mediterranean regions. The young heads are consumed as fresh or cooked artichoke-like vegetable. Land cultivation and overgrazing are 2 major thr... 展开 Gundelia tournefortii is a perennial spiny herb, and a neglected plant, found in the Mediterranean regions. The young heads are consumed as fresh or cooked artichoke-like vegetable. Land cultivation and overgrazing are 2 major threats to the presence of Gundelia, and efforts are needed to save it from loss and to have it available for consumption. This study investigated the in vitro multiplication of G. tournefortii with emphasis on the chemical factors of the culture media and ex vitro acclimatization. An experiment was conducted for in vitro germination of seeds in relation to mineral composition of the culture media, light or plant growth regulators. The best media for seed germination were agar-water and quarter-strength MS with 5.9 micro M GA3 in the dark (95 and 87% germination, respectively). Proliferation of shoots was also studied at different levels of zeatin, benzyladenine and kinetin. High concentrations of zeatin (13.7 micro M) or benzyladenine (8.9 micro M) resulted in the highest growth parameters (number of shoots, shoot height, number of leaves and callus diameter). The effect of different levels of IAA, IBA and NAA on rooting ability of microshoots was studied. The best result in terms of root number and length was achieved at 2.7 micro M NAA, 9.8 micro M IBA, or 5.7 micro M IAA. Rooting qualities were enhanced when 1.0 g activated charcoal was used in the rooting medium containing 2.7 micro M NAA. Rooted plantlets were successfully acclimatized ex vitro with 80% survival. Acclimatized plants were successfully grown in the greenhouse and did not show phenotypic variations. 收起