摘要 : The effects of the <i>Saccharomyces cerevisiae</i> extract on some organ, liver, and pancreatic digestive enzymes in breeder hens fed on aflatoxin (AF)-contaminated feed were investigated. Forty-eight 58-wk-old Ross 308 breeder he... 展开 The effects of the <i>Saccharomyces cerevisiae</i> extract on some organ, liver, and pancreatic digestive enzymes in breeder hens fed on aflatoxin (AF)-contaminated feed were investigated. Forty-eight 58-wk-old Ross 308 breeder hens were used. The hens were fed diets containing 0 or 100 micro g of AF/kg and 0 or 1 g of <i>S. cerevisiae</i>/kg in a 2x2 factorial arrangement of treatments. Although serum alkaline phosphatase levels were significantly higher, serum alkaline aminotransferase (<i>P</i>=0.068) and gamma-glutamyltransferase (<i>P</i>=0.067) levels tended to increase (<i>P</i><0.05) in hens fed the AF-contaminated diet than those of hens fed the uncontaminated diet. Both AF and <i>S. cerevisiae</i> extract increased (<i>P</i><0.001) pancreatic amylase activity, but the effect was not additive, resulting in an AF x <i>S. cerevisiae</i> extract interaction (<i>P</i><0.001). -Amylase activity in duodenum was lower (<i>P</i><0.001) in hens fed the AF-contaminated diet. Duodenum -amylase activity was higher (<i>P</i>=0.024), but jejunum -amylase activity was lower in <i>S. cerevisiae</i> extract-supplemented hens than that of nonsupplemented hens. There was a significant interaction between AF and <i>S. cerevisiae</i> extract on pancreatic and duodenal lipase activity. Pancreatic lipase activity decreased in hens fed the AF-contaminated diet. However, <i>S. cerevisiae</i> supplementation extract minimized this effect of AF on pancreatic lipase activity. Duodenal lipase activity was decreased in hens fed the AF-contaminated diet without <i>S. cerevisiae</i> extract supplementation. However, there were not any significant differences between hens fed the AF-contaminated diet and hens fed the uncontaminated diet after <i>S. cerevisiae</i> extract supplementation. Pancreatic trypsin activity was higher (<i>P</i>=0.044) in hens fed the AF-contaminated diet than that of hens fed the uncontaminated diet. There was a significant interaction between AF and <i>S. cerevisiae</i> extract on pancreatic chymotrypsin activity. It was increased in hens fed the AF-contaminated diet without <i>S. cerevisiae</i> extract supplementation. However, <i>S. cerevisiae</i> extract supplementation counteracted this negative effect of AF on pancreatic chymotrypsin activity. The treatments did not result in any change in duodenal chymotrypsin activity, but <i>S. cerevisiae</i> supplementation decreased (<i>P</i><0.05) jejunal chymotrypsin activity. In conclusion, our results showed that addition of 1 g/kg of <i>S. cerevisiae</i> extract reduces the toxic effects of AF on pancreatic lipase and chymotrypsin activity. Therefore, it may be useful to supplement feedstuff with <i>S. cerevisiae</i> extract to reduce the effects of AF in laying breeder hens. 收起