摘要 : Various concentrations of kanamycin and paromomycin were examined for their effect on embryogenic cell suspension viability, transformation efficiency, and transgenic plant regeneration. Paromomycin (10-25 mg/1) induced an earlier... 展开 Various concentrations of kanamycin and paromomycin were examined for their effect on embryogenic cell suspension viability, transformation efficiency, and transgenic plant regeneration. Paromomycin (10-25 mg/1) induced an earlier killing effect on cell suspensions than kanamycin (40-100 mg/l). Transformation efficiency and the number of embryos developed on selection medium were positively correlated with an increase in paromomycin concentrations from 10 to 20 or 25 mg/l. Paromomycin was more effective than kanamycin in selection of transformed cells and induction of embryo development during selection. Although no differences in transformation efficiency were observed between cryopreserved and non-cryopreserved cells, conversions of transformed cells to embryos and plantlets were significantly improved when cryopreserved cells were used as the target material. With the improved transformation procedure proposed, 65-80% of GUS-positive cells and embryos were produced with about 40% of transformed embryos regenerated into plants. (C) 2004 Elsevier Ireland Ltd. All rights reserved. 收起