摘要 : A novel underground reservoir was constructed in an abandoned tunnel for preserving the water in a remote island. The microbial community, potential opportunistic pathogens (OPs) and antibiotic resistance genes (ARGs) of water wer... 展开 A novel underground reservoir was constructed in an abandoned tunnel for preserving the water in a remote island. The microbial community, potential opportunistic pathogens (OPs) and antibiotic resistance genes (ARGs) of water were analyzed over a year. Results demonstrated that the culturability of the total bacteria and coliforms were both sensitive to the temperature, and the total bacteria culturability had significantly positive relationship with temperature over a year. The n-ary sumation ARGs abundance sharply decreased from September to January, but bounced back in July, which was strongly related with phylum Proteobacteria, genera Methylophilus and Methylotenera. The potential OPs almost carried a variety of ARGs, especially in genera Pseudomonas, Enterobacter, Klebsiella, Escherichia with more than 40 ARGs types, which resulted in ARGs enrichment and transfer in the water system. The n-ary sumation ARGs of potential OPs variation was extremely significantly related with ARGs subtypes of MacB (r = 0.980, p < 0.05), AcrB (r = 0.997, p < 0.01), AcrA (r = 0.986, p < 0.05) and genus Klebsiella (r = 0.962, p < 0.05). 收起