摘要 : Screening 50 greenhouse grown Jalapeno M (JM) (Capsicum annuum) pepper plants for the presence of pepper cryptic virus - 1 (PCV-1) by dsRNA extraction and analysis by electrophoresis, resulted in two JM lines. One was PCV-1 free a... 展开 Screening 50 greenhouse grown Jalapeno M (JM) (Capsicum annuum) pepper plants for the presence of pepper cryptic virus - 1 (PCV-1) by dsRNA extraction and analysis by electrophoresis, resulted in two JM lines. One was PCV-1 free and designated JM-N. The other one was PCV-1 positive and designated JM-P. Both lines were susceptible to natural infection by Cucumber mosaic virus and its satellite RNA. PCV-1 was not graft transmitted but it was transmitted both paternally (pollen) and maternally (ovule).Partial sequence of the RNA-dependent RNA polymerase gene was obtained from three overlapping cDNA clones derived from PCV-1 dsRNA. A search for aminoacid sequence similarity using the NCBI BLAST resulted in significant alignments with the RNA dependentRNA polymerase of Beet cryptic virus -3 and several putative members of the family Partitiviridae. Data presented indicate that PCV-1 is a distinct species of the family Partitiviridae. 收起