摘要 : kappa-Carrageenases belong to family 16 of glycoside hydrolases that degrade sulfated galactans in the red algae known as kappa-carrageenans. A novel kappa-carrageenase, named PLJ30, was cloned from Pseudoalteromonas carrageenovor... 展开 kappa-Carrageenases belong to family 16 of glycoside hydrolases that degrade sulfated galactans in the red algae known as kappa-carrageenans. A novel kappa-carrageenase, named PLJ30, was cloned from Pseudoalteromonas carrageenovora ASY5. PLJ30 showed the highest activity at 45 degrees C and pH 6.5. Its activity was impaired by most metal ions and reagents we tested. The ESI-MS analysis of hydrolysates showed that the degradation products are mainly disaccharides and tetrasaccharides, indicating that the enzyme is an endo-type carrageenase. It could be a valuable enzyme tool to produce carrageenan oligosaccharides. 收起