摘要 : RsmA, a ribosomal RNA small subunit methyltransferase from Staphylococcus aureus, catalyzes the N-6 methylation of adenine in 16S rRNA. In this study, RsmA from Staphylococcus aureus was cloned, expressed, purified and crystallize... 展开 RsmA, a ribosomal RNA small subunit methyltransferase from Staphylococcus aureus, catalyzes the N-6 methylation of adenine in 16S rRNA. In this study, RsmA from Staphylococcus aureus was cloned, expressed, purified and crystallized. The crystal belonged to space group C2, with unit-cell parameters a = 84.38, b = 157.76, c = 96.50 angstrom, = 95.04 degrees. X-ray diffraction data were collected to a resolution of 3.2 angstrom. The self-rotation function and the Matthews coefficient suggested the presence of two molecules in the asymmetric unit. 收起