摘要 : The formation of DNA adducts 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 1,<i>N</i>⁶-etheno-2'-deoxyadenosine ( epsilon dAdo) in organs of rainbow trout fed with different lipid sources was investigated. Fish we... 展开 The formation of DNA adducts 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 1,<i>N</i>⁶-etheno-2'-deoxyadenosine ( epsilon dAdo) in organs of rainbow trout fed with different lipid sources was investigated. Fish were fed for 16 weeks with five experimental diets supplemented either with fish oil (FO), or one of four different vegetable oils: olive oil (OO), sunflower oil (SO), linseed oil (LO), and palm oil (PO). DNA adducts were measured by LC-ESI/MS/MS in the liver, spleen and kidney to evaluate DNA damage. The highest 8-oxodGuo levels were observed in liver and kidney of fish fed the FO diet (7814+or-678 and 13,554+or-1571 adducts/10⁹ nucleosides, respectively), and in spleen of fish receiving the SO diet (13,541 adducts/10⁹ nucleosides). The PO diet resulted in the lowest content of 8-oxodGuo in all tissues, 6720+or-652 adducts/10⁹ nucleosides in liver, 8340+or-668 adducts/10⁹ nucleosides in kidney, and 6202+or-1038 adducts/10⁹ nucleosides in spleen. Similarly, epsilon dAdo was highest in the FO treatment, 12.5+or-1.9 adducts/10⁹ and 58.2+or-4.6 adducts/10⁹ nucleosides in liver and kidney respectively, whereas SO produced the highest epsilon dAdo value (568.2 adducts/10⁹ nucleosides) in spleen. In spleen and kidney, epsilon dAdo was lowest in the PO treatment (47.8 and 27.1+or-5.9 adducts/10⁹, respectively). In liver, the OO diet registered the lowest epsilon dAdo value (8.9+or-1.9 adducts/10⁹ nucleosides). Significant positive correlations were observed between levels of 8-oxodGuo in kidney tissues and the amount of 20:4n-6, 20:5n-3, 22:6n-3, n-3 LC-PUFA and n-6 LC-PUFA intake (<i>P</i><0.05). The epsilon dAdo levels in spleen tissue were correlated with 18:2n-6 and n-6 PUFA intake (<i>P</i><0.05). This study suggests that dietary lipids play an important role in the oxidatively generated damage to DNA in fish. 收起