摘要 : Small vegetative parts of <i>Aconitum balfourii</i> from different growth stages were excised for <i>in vitro</i> establishment. The callus induction was achieved from cultured leaf segment on MS medium fortified with NAA (2.5 mg/... 展开 Small vegetative parts of <i>Aconitum balfourii</i> from different growth stages were excised for <i>in vitro</i> establishment. The callus induction was achieved from cultured leaf segment on MS medium fortified with NAA (2.5 mg/1) and BAP (1 mg/1). Calli upon transfer onto MS medium containing BA alone (0.5-4 mg/l) for shoot regeneration, multiplication and elongation. Up to 25 shoots per culture flask were obtained on 2 mg/l BAP after 7-8 weeks of culture. The optimum rooting on micro-shoots was observed on MS supplemented with IAA (1.8 mg/l). The <i>in vitro</i> developed plantlets with elongated roots were then subjected for further <i>ex vitro</i> rooting, acclimatization and hardening. 收起