摘要 : Effects of some growth regulators (IAA, NAA, 2,4-D, BAP and KIN) have been studied on the regenerative potential of internodal explants in <i>Tinospora cordifolia</i> under <i>in vitro</i> conditions. Internodal explants were inoc... 展开 Effects of some growth regulators (IAA, NAA, 2,4-D, BAP and KIN) have been studied on the regenerative potential of internodal explants in <i>Tinospora cordifolia</i> under <i>in vitro</i> conditions. Internodal explants were inoculated on Murashige and Skoog (MS) and Woody Plant Medium (WPM) supplemented with different concentrations (0.5, 1.0, 2.0 and 4.0 mg/l) of these growth regulators individually. Internodal explants produced callus at all the four concentrations (0.5, 1.0, 2.0 and 4.0 mg/l) of IAA, NAA and 2,4-D on both the media. No callusing occurred in control cultures as well as on media supplemented with BAP and Kn. Callus formation increased with increase in concentration of IAA, NAA and 2,4-D from 0.5 to 4.0 mg/l. At 4.0 mg/l 2,4-D on WPM per cent response was 70% and mean fresh weight was 2.74 g. The callus was transferred to media with different concentrations of BAP (0.5, 1.0 and 2.0 and 4.0 mg/l) alone and in combination with IAA, NAA and 2,4-D for differentiation. BAP (0.5 and 2.0 mg/l) in combination with (0.5 and 4.0 mg/l) IAA, NAA and 2,4-D showed both shoot as well as root formation on WPM. 收起