摘要 : This study focused on assessing mechanism of bone marrow mesenchymal stem cells (BMSCs) with high miR-125b level on leukemia cells. Cultured MSC cells were identified, transfected with miR-125b, and miR-125b level was tested by Re... 展开 This study focused on assessing mechanism of bone marrow mesenchymal stem cells (BMSCs) with high miR-125b level on leukemia cells. Cultured MSC cells were identified, transfected with miR-125b, and miR-125b level was tested by Reverse Transcription-Polymerase Chain Reaction (RT-PCR).After transfection, cells were divided into NC group, miR-125b mimics group, miR-125b inhibitor group, BMSC group and BMSCs group with high miR-125b expression. The miR-125b target gene was further explored by luciferase activity. Morphology of BMSC cell line P3 was stable, and CD90, CD71and CD29 surface markers were positive, while CD45 was negative. miR-125b overexpression of BMSC in this study was successfully transfected, and the BMSCs with transfected miR-125b notably suppressed THP-1 cells proliferation, further enhancing cell apoptosis and levels of apoptosis-relatedproteins. Moreover, the BMSCs with miR-125b notably inhibited colony formation ability, and induced G1 phase arrest. miR-125b showed targeted relationship to Bak1, while the BMSCs with miR-125b targeted Bak1 gene, leading to up-regulation of Bakl, p53 and Puma protein expressions and inhibitionof proliferation and apoptosis of leukemia THP-1 cells. miR-125b can therefore be used as a therapeutic target for acute myeloid leukemia. 收起