摘要 : Objective:To investigate the effects of exosomes from human T lymphocyte line(H9)treated with HIV envelope protein gp120 on macrophage polarization.Methods:The viability of gp120-treated H9 T lymphocytes was as-sessed using the CC... 展开 Objective:To investigate the effects of exosomes from human T lymphocyte line(H9)treated with HIV envelope protein gp120 on macrophage polarization.Methods:The viability of gp120-treated H9 T lymphocytes was as-sessed using the CCK8 assay.Inflammatory cytokine levels in the supernatant of H9 cells were determined by ELISA.Exosome characteristics were identified through electron microscopy and Western blot experiments.PHK67 staining was employed to observe the uptake of exosomes by macrophages.Finally,macrophage polarization markers were detected using ELISA and immuno-fluorescence to analyze the impact of gp120-treated T cell exosomes on macrophage polarization.Results:HIV gp120 protein inhibited the proliferation of human T lymphocytes H9 and promoted the release of inflammatory cytokines.Exosomes from H9 T lymphocytes were successfully isolated,displaying a cup-shaped membranous structure under electron microscopy and overexpressing marker proteins CD9,CD63,and CD81.PHK67 staining results indicated that exosomes from H9 cells could be internalized by macrophages.Exosomes from gp120-treated H9 cells promoted the polarization of macrophages towards the M2 pheno-type.Conclusion:Exosomes secreted by human T lymphocytes H9 treated with HIV envelope protein gp120 can promote M2 polarization of macrophages,suggesting a potential novel mechanism of gp120 in immune modulation. 收起