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Nanozymes can mimic the activities of diverse enzymes, and this ability finds applications in analytical sciences and industrial chemistry, as well as in biomedical applications. Among the latter, prodrug conversion mediated by na...
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Nanozymes can mimic the activities of diverse enzymes, and this ability finds applications in analytical sciences and industrial chemistry, as well as in biomedical applications. Among the latter, prodrug conversion mediated by nanozymes is investigated as a step toward site-specific drug synthesis, to achieve localized therapeutic effects. In this work, we investigated a ceria nanozyme as a mimic to phosphatase, to mediate conversion of phosphate prodrugs into corresponding therapeutics. To this end, the substrate scope of ceria as a phosphatase mimic was analyzed using a broad range of natural phosphor(di)esters and pyrophosphates. Knowledge of this scope guided the selection of existing phosphate prodrugs that can be converted by ceria into the corresponding therapeutics. “Extended scaffold phosphates” were engineered using self-immolative linkers to accommodate a prodrug design for amine-containing drugs, such as monomethyl auristatin E. Phosphate prodrugs masked activity of the toxin, whereas prodrug conversion mediated by the nanozyme restored drug toxicity, which was validated in mammalian cell culture. The main novelty of this work lies in the rational pairing of the ceria nanozyme with the existing and the de novo designed “extended scaffold” phosphate prodrugs toward their use in nanozyme–prodrug therapy based on the defined nanozyme substrate scope.
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The past decade has seen a period for the rapid development of nanozymes. As a class of nanoscale materials with some enzyme-like properties, nanozymes not only possess catalytic activity comparable to that of natural enzymes with...
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The past decade has seen a period for the rapid development of nanozymes. As a class of nanoscale materials with some enzyme-like properties, nanozymes not only possess catalytic activity comparable to that of natural enzymes with the help of advanced technologies, but also have unique superiorities including excellent robustness in harsh environments, large-scale production, low cost, and ease of performance modulation. These advantages make them a promising generation of artificial enzymes for various applications. Especially in the analytical field, the nature of nanozyme catalysis offers amplified signals for high-sensitivity detection. However, most of currently developed nanozymes lack essential catalytic specificity, which becomes one of their biggest challenges in sensing. How to realize selective detection turns to be a vital problem in the nanozyme analytical community. In this review, we first summarize strategies available to make nanozymes work for the analytical application with good specificity. Then, typical examples utilizing these strategies to achieve selective detection are introduced, followed by the discussion of their merits as well as challenges. In the end, trends of nanozymes in this promising area are presented, to provide some guides for designing nanozyme sensing methods and devices for practical applications. (C) 2021 Elsevier B.V. All rights reserved.
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We construct a peptide-conjugated metal cluster as an enzyme-like catalytic bioprobe to enhance quantitative analysis of a membrane protein biomarker and detect epithelial-to-mesenchymal transition of tumor cells. This bioprobe wi...
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We construct a peptide-conjugated metal cluster as an enzyme-like catalytic bioprobe to enhance quantitative analysis of a membrane protein biomarker and detect epithelial-to-mesenchymal transition of tumor cells. This bioprobe with atomically precise formula, termed clusterzyme, possesses selective recognition and intrinsic enzyme-like activity. These favorable features facilitate sensitive quantitative analysis of the membrane protein in situ through on-cell catalytic signal amplification. This clusterzyme-based analytical method exhibits excellent compatibility with a traditional enzyme-linked immunosorbent assay and improved detection sensitivity with accuracy and robustness. Further, the expression level of the membrane protein reflects the ability of migration and invasion of model tumor cells, revealing epithelial-to-mesenchymal transition process. This work offers a facile and sensitive approach to monitor tumor cell type evolution at the molecular level, demonstrating a potential application of early cancer diagnosis and therapy assessment.
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We construct a peptide-conjugated metal cluster as an enzyme-like catalytic bioprobe to enhance quantitative analysis of a membrane protein biomarker and detect epithelial-to-mesenchymal transition of tumor cells. This bioprobe wi...
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We construct a peptide-conjugated metal cluster as an enzyme-like catalytic bioprobe to enhance quantitative analysis of a membrane protein biomarker and detect epithelial-to-mesenchymal transition of tumor cells. This bioprobe with atomically precise formula, termed clusterzyme, possesses selective recognition and intrinsic enzyme-like activity. These favorable features facilitate sensitive quantitative analysis of the membrane protein in situ through on-cell catalytic signal amplification. This clusterzyme-based analytical method exhibits excellent compatibility with a traditional enzyme-linked immunosorbent assay and improved detection sensitivity with accuracy and robustness. Further, the expression level of the membrane protein reflects the ability of migration and invasion of model tumor cells, revealing epithelial-to-mesenchymal transition process. This work offers a facile and sensitive approach to monitor tumor cell type evolution at the molecular level, demonstrating a potential application of early cancer diagnosis and therapy assessment.
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Zearalenone (ZEN) is a widespread nonsteroidal mycotoxin with estrogen-like activity. Sensitive and reliable quantification of ZEN in food is critical to ensure food safety and safeguard agricultural production. Herein, by combini...
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Zearalenone (ZEN) is a widespread nonsteroidal mycotoxin with estrogen-like activity. Sensitive and reliable quantification of ZEN in food is critical to ensure food safety and safeguard agricultural production. Herein, by combining the metal-organic frameworks-based nanozyme (MOFzyme) and hyaluronic acid (HA)-DNA hydrogel, a well-designed colorimetric aptasensor was developed. The HA-DNA hydrogel was deposited on the surface of the bimetallic MOFzyme via strand-induced hybridization chain reaction (HCR). Relying on the ZEN-specific aptamer integrated in hydrogel structure, the disintegration of the hydrogel network and the concomitant exposure of the encapsulated MOFzyme could be specifically triggered by the introduction of ZEN molecules. Moreover, the magnitude of hydrogel disintegration was positively correlated with the amount of ZEN, by which the quantification of ZEN can be effortlessly achieved. Benefiting from the delicate design, the satisfactory catalytic performance and stability of bimetallic MOFzyme and the appealing stimuli-responsiveness of DNA hydrogel, the developed aptasensor demonstrated superior analytical performance and ease of use. Under optimal conditions, the linear range of the aptasensor fell between 0.001 and 200 ng mL-1 with a limit of detection (LOD) of 0.8 pg mL-1. Furthermore, the aptasensor was successfully applied for the quantitative detection of ZEN in corn and soybean samples with recoveries ranging from 94.0% to 109.0%. The developed aptasensor is expected to be a prospective universal platform for accurately quantifying food or environmental hazards.
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Copper aspartate nanofibers were facilely prepared based on aspartic acid and copper (CuAsp nanofibers). It is found that the prepared CuAsp nanofibers have catalytic activities of five enzymes, including peroxidase, laccase, cata...
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Copper aspartate nanofibers were facilely prepared based on aspartic acid and copper (CuAsp nanofibers). It is found that the prepared CuAsp nanofibers have catalytic activities of five enzymes, including peroxidase, laccase, catalase, ascorbate oxidase, and superoxide dismutase mimetic activities. The kinetic and catalytic properties of CuAsp nanofibers were systematically investigated, showing their high catalytic activity, excellent stability, and reusability. The laccase mimetic activity of nanofibers could be used to detect catechin in the range 20-1200 mu M with a detection limit of 5.88 mu M. In addition, a sensing platform for glutathione with a detection limit of 0.25 mu M and a detection range of 1-50 mu M was established based on CuAsp nanofibers which have the peroxidase-mimicking activity. The sensor had good selectivity and could detect glutathione in actual samples of human serum. Therefore, CuAsp nanofibers with multi-enzyme activity have broad application prospects such as biosensing, environmental management, and disease diagnosis.
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Carbon based nanozyme is gaining increasingly attentions due to its high stability and tailorable architecture. However, the practical applications are largely restricted by its moderate catalytic activity. External field stimulat...
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Carbon based nanozyme is gaining increasingly attentions due to its high stability and tailorable architecture. However, the practical applications are largely restricted by its moderate catalytic activity. External field stimulation and heteroatom doping are two effective strategies for improving enzyme mimic activities. Here, sulfur doped graphdiyne (SGDY) nanosheets were synthesized using a straightforward one-pot hydrothermal approach and reveals impressive peroxidase-like activities as well as piezoelectric properties. Kinetic investigations show that ultrasound irradiation boosts the peroxidase-like activities of SGDY. DFT calculations explicate the mechanism of piezoelectric effect on promoting peroxidase-like activity. The degradation of dyes and antibiotics, as well as antibacterial studies, demonstrate that SGDY has the great potential to be utilized in wastewater treatment. In addition, a laboratory scale device for continuous wastewater treatment was developed as a proof of concept for the practical applications of piezoelectric enhanced nanozymes. This work not only sheds light on the mechanism of nanozyme-/piezocatalysis coupling process, but also opens up a new avenue for the rational design of nanozymes with high activity for wastewater treatment and bacterial disinfection.(c) 2022 Elsevier Ltd. All rights reserved.
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Abstract How to establish a multi‐modality oncotherapy method on the basis of single matter mediated utilization of tumor endogenous substances is very promising for efficient antitumor treatment. In this work, we have designed a...
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Abstract How to establish a multi‐modality oncotherapy method on the basis of single matter mediated utilization of tumor endogenous substances is very promising for efficient antitumor treatment. In this work, we have designed a metallic Ni0.5Fe0.5S2 nanosphere for synergetic chemodynamic/photodynamic/photothermal antitumor treatments. Under NIR excitation, a remarkable photothermal effect could be generated by Ni0.5Fe0.5S2 for realizing the photothermal therapy. Meanwhile, Ni0.5Fe0.5S2 could also trigger formation of reactive oxygen species (ROS) via transformation of tumor‐site oxygen for the further photodynamic therapy. Furthermore, peroxidase‐like property of Ni0.5Fe0.5S2 favors the catalysis of endogenous H2O2 into hydroxyl radical to achieve a chemodynamic therapy. As a result, the on‐site tumor hyperthermia, tumor‐site oxygen and H2O2 conversion are worked for the antitumor action. On the merits of above synergistic effect, a better antitumor outcome was confirmed as compared with any single antitumor action, and complete removal of solid tumor under a relative lower temperature has been accomplished.
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Lately,gold nanoparticles have shown great compound mirroring exercises which take after those of peroxidase,oxidase,catalase,superoxide dismutase or reductase.This,converged without breaking a sweat of amalgamation,tunabiliry,bio...
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Lately,gold nanoparticles have shown great compound mirroring exercises which take after those of peroxidase,oxidase,catalase,superoxide dismutase or reductase.This,converged without breaking a sweat of amalgamation,tunabiliry,biocompatibility and minimal expense,makes them fantastic up-and-comers when contrasted and natural compounds for applications in biomedicine or biochemical investigations.In this,more than 200 exploration papers have been methodically assessed to introduce the new advancement on the essentials of gold nanozymes and their possible applications.The survey uncovers that the morphology and surface science of the nanoparticles assume a significant part in their reactant properties,just as outside boundaries like pH or temperature.However,genuine applications regularly require explicit biorecognition components to be immobilized onto the nanozymes,prompting surprising positive.
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Though nanozymes are successfully applied in various areas, the increasing demands facilitate the exploitation of nanozymes possessing higher activity and more functions. Natural enzyme-linked receptors (ELRs) are critical compone...
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Though nanozymes are successfully applied in various areas, the increasing demands facilitate the exploitation of nanozymes possessing higher activity and more functions. Natural enzyme-linked receptors (ELRs) are critical components for signal transductions in vivo by expressing activity variations after binding with ligands. Inspired by this, the defect-engineered carbon nitrides (DCN) are reported to serve as nanozyme-linked receptors (NLRs). For one thing, cyano defects increase the enzyme-like activity by a factor of 109.5. For another, DCN-based NLRs are constructed by employing cyano groups as receptors, and variable outputs are ensued upon the addition of ion ligands. Significantly, both the cascade effect and electronic effect are demonstrated to contribute to this phenomenon. Finally, NLRs are used for pattern recognition of metal ions, indicating the signal transduction ability of NLRs as well. This work not only provides great promise of defect engineering in nanozymes, but also contributes to the design of artificial ELRs.
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