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Fluorescence imaging is a powerful technique for visualizing temporal and spatial changes of biological events in living samples by utilizing fluorescent probes whose properties are altered upon interaction with the target biomole...
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Fluorescence imaging is a powerful technique for visualizing temporal and spatial changes of biological events in living samples by utilizing fluorescent probes whose properties are altered upon interaction with the target biomolecule. In the past few years, many fluorescent probes have been developed based on xanthene dyes in which the O atom at the 10-position is replaced with a Si atom, such as 2,7-bis(dimethylamino)9,9-dimethyl- 9-sila-9H-anthracenium (TMDHS), Si-rhodamines and TokyoMagentas. Interestingly, these fluorophores not only retain the advantages of the typical xanthene dyes, but also have a number of unique properties, including far-red to nearinfrared emission. This review summarizes silicon-substituted xanthene dyes reported to date, along with their photophysical properties. We include examples of applications of probes utilizing these fluorophores, such as multi-color imaging and super-resolution live-cell microscopy.
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We present single-molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single-molecule fluorescence experiments proved mCherry as an ideally suited fluorophore fo...
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We present single-molecule fluorescence data of fluorescent proteins GFP, YFP, DsRed, and mCherry, a new derivative of DsRed. Ensemble and single-molecule fluorescence experiments proved mCherry as an ideally suited fluorophore for single-molecule applications, demonstrated by high photostability and rare fluorescence-intensity fluctuations. Although mCherry exhibits the lowest fluorescence quantum yield among the fluorescent proteins investigated, its superior photophysical characteristics suggest mCherry as an ideal alternative in single-molecule fluorescence experiments. Due to its spectral characteristics and short fluorescence lifetime of 1.46 ns, mCherry complements other existing fluorescent proteins and is recommended for tracking and localization of target molecules with high accuracy, fluorescence resonance energy transfer (FRET), fluorescence lifetime imaging microscopy (FLIM), or multicolor applications.
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In this work, a novel sodium-fluorescent crystal (Na-FS) was synthesized from 4-dimethylaminobenzoic acid and sodium hydroxide by one-pot hydrothermal method. The structure and conformation of Na-FS were confirmed by single-crysta...
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In this work, a novel sodium-fluorescent crystal (Na-FS) was synthesized from 4-dimethylaminobenzoic acid and sodium hydroxide by one-pot hydrothermal method. The structure and conformation of Na-FS were confirmed by single-crystal X-ray diffraction and scanning electron microscope, and the optical properties were studied by fluorescence spectrometer. The results showed that: Na-FS was a triclinic crystal, space group was P-1, cell parameters a , b and c were 10.5113(3), 15.9198(5) and 15.9560(5) ?, respectively, and the number of independent atoms Z in a structure cell was two. Additionally, Na-FS has a blue fluorescence emission (around 360 nm under excited at the range of 230–300 nm) with great photostability and photobleaching resistance, and the quantum yield of Na-FS is 30.58%.
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A novel dual channel mitochondria-targeted fluorescent probe has been developed to detect nitric oxide (NO), an important and short-life reactive nitrogen species (RNS) which mainly generates in the mitochondria. The new probe Mi-...
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A novel dual channel mitochondria-targeted fluorescent probe has been developed to detect nitric oxide (NO), an important and short-life reactive nitrogen species (RNS) which mainly generates in the mitochondria. The new probe Mi-NO has exhibited dual emission property as well as unique spectrum responses towards NO. Upon the addition of NO, the fluorescence of Mi-NO quenched significantly. Molecular docking calculations suggest a potential molecular target for Mi-NO in the mitochondria protein adenine nucleotide translocator (ANT). High colocalization coefficient also suggested that Mi-NO can effectively target mitochondria. Moreover, Mi-NO has been successfully employed in imaging NO in living cells and zebrafish, affording a potential for studying the biological functions of NO in living systems.
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For practical application of plant fluorescence measurement as a plant monitoring method, time required for measurement should be minimized. If measurement is conducted at night, one minute measurement per one point will be within...
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For practical application of plant fluorescence measurement as a plant monitoring method, time required for measurement should be minimized. If measurement is conducted at night, one minute measurement per one point will be within an acceptable range. Another problem in applying this method in plant production settings is the spatial variations of the fluorescence characteristics within a plant. To elucidate spatial variations, one minute fluorescence measurment was conducted at ten different points within Rhododendron pulchrum. The spectra and fluorescence induction kinetics within one minute reflected the light environment each leaf has experienced.
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Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared, where cellular ...
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Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared, where cellular autofluorescence is minimized. In the present study, carbopyronines absorbing around 640 nm and emitting at around 660 nm, with a low intersystem crossing rate (k_(isc) ≈ 0.5×10~6 s~(-1)) and excellent properties for cellular imaging were synthesized. A general synthetic route to carbopyronines with functional groups variable in the final steps of the synthesis or in the resulting fluorescent dye is presented. Possessing two 2-me-thoxyethyl groups, the parent dye is soluble in water and most organic solvents. Demethylation of the dye or its precursors is straightforward, clean, and furnishes compounds with one or two 2-hydroxyethyl groups, which can be used for further transformations. Modifications in the linker-containing carboxy group are also possible. A multistep synthesis of the dye starting from a simple precursor and utilizing a single temporary protective group is described. The presented approach may be further applied to the design of caged carbopyronines.
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Synergistic evolution of fluorescent Au(I)@(Ag_2/Ag_3)-thiolate core?shell particles has been made possible under the Sun in presence of the respective precursor coinage metal compounds and glutathione (GSH). The green chemically ...
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Synergistic evolution of fluorescent Au(I)@(Ag_2/Ag_3)-thiolate core?shell particles has been made possible under the Sun in presence of the respective precursor coinage metal compounds and glutathione (GSH). The green chemically synthesized fluorescent clusters are giant (～600 nm) in size and robust. Among all the common water miscible solvents, exclusively DMSO exhibits selective fluorescence quenching (Turn Off) because of the removal of GSH from the giant cluster. Again, only Pb(II) ion brings back the lost fluorescence (Turn On) leaving aside all other metal ions. This happens owing to the strong affinity of the sulfur donor of DMSO for Pb(II). Thus, employing the aqueous solution containing the giant cluster, we can detect DMSO contamination in water bodies at trace level. Besides, a selective sensing platform has emerged out for Pb(II) ion with a detection limit of 14 × 10~(?8) M. Pb(II) induced fluorescence recovery is again vanished by I~? implying a promising route to sense I~? ion.
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The fluorescent responses of 3,3'-di(trifluoroacetyl)-1,1'-bi- 2-naphthol toward a variety of amines have been studied. It was found that the aliphatic primary 1,2- and 1,5-diamines can greatly enhance the fluorescence of this com...
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The fluorescent responses of 3,3'-di(trifluoroacetyl)-1,1'-bi- 2-naphthol toward a variety of amines have been studied. It was found that the aliphatic primary 1,2- and 1,5-diamines can greatly enhance the fluorescence of this compound, but under the same conditions, primary, secondary, and tertiary monoamines cannot turn on the fluorescence of this compound. In addition, this compound was shown to be an enantioselective and diastereoselective fluorescent sensor for chiral diamines. UV absorption and NMR spectroscopic methods have been used to study the interaction of the sensor with amines. These studies have demonstrated that the intramolecular OH···O=C hydrogen bonding of the sensor is important for both the reactivity of its trifluoroacetyl group with the amines and its fluorescent responses. The interaction of both of the two amine groups of a diamine molecule with the sensor is essential for the observed fluorescent sensitivity and selectivity.
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The majority of dissolved organic matter (DOM) in the ocean is resistant to microbial degradation, yet its formation remains poorly understood. The fluorescent fraction of DOM can be used to trace the formation of recalcitrant DOM...
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The majority of dissolved organic matter (DOM) in the ocean is resistant to microbial degradation, yet its formation remains poorly understood. The fluorescent fraction of DOM can be used to trace the formation of recalcitrant DOM (RDOM). A long-term (> 1 year) experiment revealed 27–52% removal of dissolved organic carbon and a nonlinear increase in RDOM fluorescence associated with microbial turnover of semilabile DOM. This fluorescence was also produced using glucose as the only initial carbon source, suggesting that degradation of prokaryote remnants contributes to RDOM. Our results indicate that the formation of a fluorescent RDOM component depends on the bioavailability of the substrate: the less labile, the larger the production of fluorescent RDOM relative to organic carbon remineralized. The anticipated increase in microbial carbon demand due to ocean warming can potentially forcemicrobes to degrade less labile substrates, thereby increasing RDOM production and stimulating ocean carbon storage.
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A brief history of the development of the method of fluorescent probes and examples of its appli- cation are presented. Works done at the 2nd Moscow medical institute and institute of physical chemical medicine in collaboration wi...
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A brief history of the development of the method of fluorescent probes and examples of its appli- cation are presented. Works done at the 2nd Moscow medical institute and institute of physical chemical medicine in collaboration with other institutes on: (1) detection of T- and B-lymphocytes in immune pathol- ogy; (2) investigation of the structure and clinical estimation of lipoproteins from blood plasma or serum in relation to the assessment of risk factors for the development of cardiovascular diseases; (3) detection of changes in album molecule in a series of pathological processes improving the prognosis of the development of such diseases as peritonitis, pancreatitis, poisoning with psychotropic preparations etc.; (4) intravital mea- surement of the potentials in electric fields in leukocytes and changes of these fields in the course of immu- nological diseases are described. With these approaches it is possible to study molecular events in the course of pathogenesis and also obtain diagnostically significant information on physical chemical aspect of these events. This information is not a conventional method used in the clinical laboratory.
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