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Abstract Core Facilities and Technology Platforms are increasingly important components of the science research landscape. However, data on facility operations and staff careers are lacking to inform their development. Here we hav...
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Abstract Core Facilities and Technology Platforms are increasingly important components of the science research landscape. However, data on facility operations and staff careers are lacking to inform their development. Here we have surveyed 114 people working in 46 light microscopy (LM) facilities within the United Kingdom. Our survey explores issues around career progression, facility operations and funding. The data show that facilities are substantial repositories of equipment and knowledge which adapt to meet the needs of their local environments. Our report highlights the challenges faced by facility managers, institutions and funders in evaluating facility performance and devising strategies to maximise the return on research funding investment.
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摘要 :AbstractThe organic paramagnetic compounds nitroxides have great potential as magnetic resonance imaging (MRI) contrast agents. Herein, we report the synthesis and characterization of glucose modified carbon quantum dot containing...
展开AbstractThe organic paramagnetic compounds nitroxides have great potential as magnetic resonance imaging (MRI) contrast agents. Herein, we report the synthesis and characterization of glucose modified carbon quantum dot containing 2,2,6,6-tetramethyl-piperidinooxy (TEMPO) for targeted bimodal MR/optical imaging of tumor cells. CQD-TEMPO-Glu shows the greatest potentials for bioimaging applications in view of low cytotoxicity, good biocompatibility, green fluorescence emission and high T1 relaxivities. Thein vitroMR and optical imaging results confirm enhanced cellular internalization of CQD-TEMPO-Glu in cancer cells through GLUT mediated endocytosis. These results confirm that CQD-TEMPO-Glu is expected to be widely exploited as dual-modal contrast for cancer imaging.Graphical abstractGlucose modified carbon quantum dot containing TEMPO (CQD-TEMPO-Glu) is expected to be exploited as targeted dual-modal contrast for cancer imaging, in view of low cytotoxicity, good biocompatibility, green fluorescence emission and high T1 relaxivities.Display Omitted收起
摘要 :AbstractThe organic paramagnetic compounds nitroxides have great potential as magnetic resonance imaging (MRI) contrast agents. Herein, we report the synthesis and characterization of glucose modified carbon quantum dot containing...
展开AbstractThe organic paramagnetic compounds nitroxides have great potential as magnetic resonance imaging (MRI) contrast agents. Herein, we report the synthesis and characterization of glucose modified carbon quantum dot containing 2,2,6,6-tetramethyl-piperidinooxy (TEMPO) for targeted bimodal MR/optical imaging of tumor cells. CQD-TEMPO-Glu shows the greatest potentials for bioimaging applications in view of low cytotoxicity, good biocompatibility, green fluorescence emission and high T1 relaxivities. Thein vitroMR and optical imaging results confirm enhanced cellular internalization of CQD-TEMPO-Glu in cancer cells through GLUT mediated endocytosis. These results confirm that CQD-TEMPO-Glu is expected to be widely exploited as dual-modal contrast for cancer imaging.Graphical abstractGlucose modified carbon quantum dot containing TEMPO (CQD-TEMPO-Glu) is expected to be exploited as targeted dual-modal contrast for cancer imaging, in view of low cytotoxicity, good biocompatibility, green fluorescence emission and high T1 relaxivities.Display OmittedHighlights•Glucose modified carbon quantum dot containing TEMPO is prepared.•CQD-TEMPO-Glu exhibits low cytotoxicity and good biocompatibility.•CQD-TEMPO-Glu shows green fluorescence emission and high T1 relaxivities.•GLUT mediated endocytosis enhances cellular internalization of CQD-TEMPO-Glu.收起
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Gold nanorods (Au NRs) have attracted extensive research interest due to their unique optical properties, adjustable aspect ratio, and easy surface modification in the fields of biosensing, bioimaging, and disease diagnosis and de...
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Gold nanorods (Au NRs) have attracted extensive research interest due to their unique optical properties, adjustable aspect ratio, and easy surface modification in the fields of biosensing, bioimaging, and disease diagnosis and detection. In this review, we present a comprehensive review of various methods for preparing gold nanorods including hard template method, electrochemistry method, photochemistry method, seed-mediated growth method, secondary growth method, and amorphous seed method. The unique optical properties of the gold nanorods and its applications in biomedical, detection, catalysis, and information storage will also be discussed in detail.
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Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful technique for the determination of metal and nonmetal distributions within biological systems with high sensitivity. An ima...
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Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful technique for the determination of metal and nonmetal distributions within biological systems with high sensitivity. An imaging LA-ICP-MS technique for Fe, Cu, Zn, and Mn was developed to produce large series of quantitative element maps in native brain sections of mice subchronically intoxicated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) as a model of Parkinson’s disease. Images were calibrated using matrix-matched laboratory standards. A software solution allowing a precise delineation of anatomical structures was implemented. Coronal brain sections were analyzed crossing the striatum and the substantia nigra, respectively. Animals sacrificed 2 h, 7 d, or 28 d after the last MPTP injection and controls were investigated. We observed significant decreases of Cu concentrations in the periventricular zone and the fascia dentata at 2 h and 7d and a recovery or overcompensation at 28 d, most pronounced in the rostral periventricular zone (+40%). In the cortex Cu decreased slightly to -10%. Fe increased in the interpeduncular nucleus (+40%) but not in the substantia nigra. This pattern is in line with a differential regulation of periventricular and parenchymal Cu, and with the histochemical localization of Fe, and congruent to regions of preferential MPTP binding described in the rodent brain. The LA-ICP-MS technique yielded valid and statistically robust results in the present study on 39 slices from 19 animals. Our findings underline the value of routine micro-local analytical techniques in the life sciences and affirm a role of Cu availability in Parkinson’s disease.
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Multiphoton excited fluorescent probes with highly emissive, photostable, low cytotoxic properties are very important for photodynamic therapy, sensing, and bioimaging, etc., even though still challenging. Here, we report the synt...
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Multiphoton excited fluorescent probes with highly emissive, photostable, low cytotoxic properties are very important for photodynamic therapy, sensing, and bioimaging, etc., even though still challenging. Here, we report the synthesis and spectroscopic studies of two statistical Zn(II)- coordinated copolymers containing different donor types and the same acceptor type (a dithienylbenzothiadiazole-based ditopic terpyridine ligand), aiming to achieving efficient multiphoton harvesting systems. Our results indicate that an angular carbazole-based ditopic terpyridine ligand donor shows a strong tendency to form a twisted intramolecular charge transfer (TICT) state. Taking advantage of the large multiphoton absorption coefficient in the donor and efficient Fo?rster resonance energy transfer (FRET) mediated by TICT state, efficiently enhanced fluorescence from the acceptor under two- and even three-photon excitation is consequently achieved. In contrast, for a linear carbazole-based ditopic terpyridine ligand donor, the enhanced multiphoton excited fluorescence from the acceptor originates from reabsorption effect instead of FRET. For the first time, we have reported the multiphoton harvesting properties of metal?organic complexes, especially stressing the crucial role of TICT state in multiphoton excited FRET, which sheds light on how to design efficient multiphoton harvesting systems in general.
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Successful development of ultrasensitive constructs for bioimaging and biosensing is a challenging task.Recently, viruses have drawn increasing attention due to their exquisite three-dimensional structuresand unique properties, in...
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Successful development of ultrasensitive constructs for bioimaging and biosensing is a challenging task.Recently, viruses have drawn increasing attention due to their exquisite three-dimensional structuresand unique properties, including multivalency, orthogonal reactivities, and responsiveness to geneticmodifications. With such well-characterized structures, functional units, such as imaging and bindingmotifs, can be engineered on the surface of viruses in a programmable, polyvalent manner, which leadsto novel nanosized sensing/imaging systems with enhanced signaling and targeting performance. This review highlights some recent progress in the applications of viruses in bioimaging and biosensing.
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We herein developed a new class of carborhodamines (CRs), i.e. 10-methoxy-substituted carborhodamines MCRs, by a simple synthesis procedure, which have absorption and emission wavelengths longer than classical CRs while retaining ...
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We herein developed a new class of carborhodamines (CRs), i.e. 10-methoxy-substituted carborhodamines MCRs, by a simple synthesis procedure, which have absorption and emission wavelengths longer than classical CRs while retaining their excellent photophysical properties. Based on the MCR platform, we constructed the mitochondria-targeted fluorescent probe MCR-DMA and demonstrated its potential for sensing singlet oxygen (~1O_2) in living cells during the photodynamic therapy process.
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Bioimaging is a fundamental technique for research in the life sciences, and it is used routinely in the medical industry. The procedure itself encompasses many different methods, such as electron microscopy, x-ray tomography, and...
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Bioimaging is a fundamental technique for research in the life sciences, and it is used routinely in the medical industry. The procedure itself encompasses many different methods, such as electron microscopy, x-ray tomography, and magnetic resonance imaging, which are becoming increasingly popular. However, optical microscopy has advantages over these other methods: It is versatile and relatively inexpensive. For these reasons, fluorescence microscopy remains an essential tool for bioimaging in research laboratories1.
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We provide experimental evidence supporting the vectorial theory for determining electric field at and near the geometrical focus of a cylindrical lens. This theory provides precise distribution of field and its polarization effec...
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We provide experimental evidence supporting the vectorial theory for determining electric field at and near the geometrical focus of a cylindrical lens. This theory provides precise distribution of field and its polarization effects. Experimental results show a close match (≈ 95% using v2-test) with the simulation results (obtained using vectorial theory). Light-sheet generated both at low and high NA cylindrical lens shows the importance of vectorial theory for further development of light-sheet techniques. Potential applications are in planar imaging systems (such as, SPIM, IML-SPIM, imaging cytometry) and spectroscopy.
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