摘要 : In this paper, we compared three vitrification-based cryopreservation techniques, viz vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos Viability of somatic embryos w... 展开 In this paper, we compared three vitrification-based cryopreservation techniques, viz vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos Viability of somatic embryos was evaluated by measuring electrolyte leakage and by regrowth on recovery medium Droplet-vitrification was the most efficient technique Optimal conditions included loading with a solution containing 1..5 M glycerol and 0 3 M sucrose for 30 min at 25°C, treatment with the PVS2 solution for 20-40 min at 0°C followed by rapid immersion in liquid nitrogen of clumps of somatic embryos placed in microdroplets of cryoprotectant solution Under such conditions, viability of cryopreserved somatic embryos reached 55 %. 收起