摘要 : A protocol for efficient plant regeneration of I. pumila was developed via somatic embryogenesis and/or organogenesis from suspension cultures. Induction of embryogenic calluses was achieved by leaf-base culture of in vitro grown ... 展开 A protocol for efficient plant regeneration of I. pumila was developed via somatic embryogenesis and/or organogenesis from suspension cultures. Induction of embryogenic calluses was achieved by leaf-base culture of in vitro grown plants on solid Murashige and Skoog (MS) medium supplemented with 3% sucrose and (in mg/litre): inositol [myo-inositol], 100; pantothenic acid, 10; nicotinic acid, 5; vitamin B1 [thiamin], 2; vitamin B6 [pyridoxine], 1; casein hydrolysate, 250; proline, 250; 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (1.0 mg/litre each). Cell suspensions were established and maintained in MS liquid medium with the same content of 2,4-D and kinetin as used for induction and proliferation of embryogenic calluses. After three subcultures, stable suspension cultures were successfully established and maintained by subculturing every 3 weeks. The suspension cultures were initially composed of single cells, bi-, three and multicellular proembryos and cell aggregates. In prolonged suspension cultures (6-8 weeks), three types of embryogenic calluses were observed: yellow, compact (type I); yellow-green, friable (type II) and white, friable (type III). The effect of cytokinins (zeatin at 0.05, 0.1, 0.2 mg/litre and/or benzyladenine at 0.1and 1.0 mg/litre, respectively) on plant regeneration of these three types of calluses were investigated. Friable (types II and III) suspension derived calluses had the highest morphogenic potential. During the regeneration process, two different regeneration pathways were observed: somatic embryogenesis and/or organogenesis dependent on used cytokinin. Germination of normally developed somatic embryos was achieved on MS solid medium without plant growth regulators. Potted plants of I. pumila grew normally and flowered. 收起