摘要 : The chromosome 1 with bigger satellite, supposed to be the W chromosome, was microdissected from the metaphase spreads of female ginkgo root-tip cells with a fine glass needle controlled by a micromanipulator. The dissected chromo... 展开 The chromosome 1 with bigger satellite, supposed to be the W chromosome, was microdissected from the metaphase spreads of female ginkgo root-tip cells with a fine glass needle controlled by a micromanipulator. The dissected chromosome was amplified invitro by the Sau3A linker adaptor mediated PCR (LA-PCR) technique. Southern hybridization analysis indicated that DNA from the single W chromosome was successfully amplified. FISH analyses with the PCR products have been performed in the metaphase spreads of female and male ginkgo. FISH signals were observed along the entire W chromosome, while along about 1/2 length at the end of the long arm of the Z chromosome, the intensity of signals was lower than at other segments. This suggested that the chromosome 1 of ginkgo might harbor different DNA sequence structures at the 1/2 length end of the long arm, and this region on the W chromosome might be a female-specific region which formed during evolution of the sex chromosomes. 收起