摘要 : The acquisition, incubation and transmission of Watermelon silver mottle virus (WSMoV) by T. palmi were investigated by examining symptoms developed on Benincasa hispida. An acquisition threshold period of 30 minutes was required ... 展开 The acquisition, incubation and transmission of Watermelon silver mottle virus (WSMoV) by T. palmi were investigated by examining symptoms developed on Benincasa hispida. An acquisition threshold period of 30 minutes was required when 5 first-instar nymphs as a group were used to transmit the virus. The efficiency of transmission increased by 2-fold when the acquisition access period increased to 24 h. Nymphs were able to transmit WSMoV within 2 h after a 2-h acquisition access feeding on diseased plants. Of the 64 thrips given a 6-h acquisition feeding at the nymph stage, approximately 63% transmitted the virus at either nymph or adult stage, or at both stages. Sixteen thrips transmitted the virus on the 1st day. Among the transmitters, approximately one fifth of the insects transmitted only once in their lifetime, and, 22, 5 and 3 individuals transmitted WSMoV 2, 3, and 4 times in their lifetime, respectively. Approximately 30, 15 and 22% thrips transmitted the virus only at the nymph stage, only at the adult stage, and at both stages, respectively. Approximately 25% of the transmitters were able to make a successive 2-day transmission. The results showed that once becoming viruliferous, T. palmi may or may not transmit WSMoV later in life. The results also showed an erratic pattern of WSMoV transmission by T. palmi. Our observations suggest that there may be two mechanisms involved in the transmission of WSMoV by T. palmi, a non-circulative styler-borne transmission at the very early stage and the other the propagative-circulative form at a later stage.. 收起