摘要 : Three different Pax6 promoters -- P0, P1, and P#alpha# -- show differential activity in the developing eye and spinal cord. To examine promoter usage during forebrain development, we performed in situ hybridization and reverse tra... 展开 Three different Pax6 promoters -- P0, P1, and P#alpha# -- show differential activity in the developing eye and spinal cord. To examine promoter usage during forebrain development, we performed in situ hybridization and reverse transcription-polymerase chain reaction to detect transcripts initiated from each promoter. Promoter-specific transcripts are expressed within subdomains of total Pax6 expression, but differ from one another in their spatial localization and expression over time. Additionally,we identified a novel P0-initiated transcript and detected a developmentally regulated antisense transcript. 收起