摘要 : Parasitic weeds such as <i>Phelipanche</i> and <i>Orobanche</i> are obligate holoparasites that attack roots of almost all economically important crops in semiarid regions of the world. A wide variety of parasitic weed control str... 展开 Parasitic weeds such as <i>Phelipanche</i> and <i>Orobanche</i> are obligate holoparasites that attack roots of almost all economically important crops in semiarid regions of the world. A wide variety of parasitic weed control strategies (chemical, biological, cultural, and resistant crops) has been tried. Unfortunately, most are partially effective and have significant limitations. The current mini review will discuss the needs for alternative methods and will summarize current and new biotechnology-based approaches for broomrape control. At present, we have generated transgenic tobacco plants expressing a cecropin peptide (<i>sarcotoxin IA</i>), under the control of the inducible <i>HMG2</i> promoter. Transgenic lines enhanced host resistance to the parasitic weed; transgenes showed higher numbers of aborted parasitization events, reduced <i>Phelipanche</i> biomass, and increased host biomass. <i>Sarcotoxin IA</i> had no obvious effect on growth and development of transgenic host plants. Mannitol content in the parasite is regulated by Mannose 6-Phosphate Reductase (<i>M6PR</i>) gene, an essential process to broomrape species for water and nutrient uptake from the host. In our study, we used the inverted repeat technique to silence the parasite target gene, <i>M6PR</i>. In this study it was shown that the endogenous <i>M6PR</i> mRNA from <i>P. aegyptiaca</i> tubercles or shoots grown on transgenic tomato plants harboring the <i>M6PR</i> silencing construct was reduced by 60 to 80%. The number of dead tubercles was also increased significantly on transgenic plants as compared with the control plants. The strategies presented here are potentially superior to other methods in that they are effective, have a low cost of implementation for producers, and are safe for the environment. 收起