摘要 : This paper describes a protocol for the efficient vegetative propagation of <i>Cleome rosea</i> by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of <i>C. rosea</i> were cultivated on Murashige and Skoo... 展开 This paper describes a protocol for the efficient vegetative propagation of <i>Cleome rosea</i> by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of <i>C. rosea</i> were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), a-naphthaleneacetic acid (NAA), 4-amino-3,5,6-trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D). Nodular calli were produced from both explant types in the presence of 4.5 and 9.0 micro M 2,4-D. Embryo development and maturation were achieved when calli from stem explants were transferred to media containing a ten-fold reduction of 2,4-D concentration initially used (0.45 and 0.90 micro M). Leaf-derived calli did not form embryos with the same treatments. The highest frequency of embryogenic callus formation (85%) and number of embryo per callus (13.45+or-2.8) were achieved during the first subculture on medium supplemented with 0.90 micro M 2,4-D. Embryo conversion into plantlets was achieved following transfer to growth regulator-free MS medium solidified with 2 g.L^-1 phytagel. An acclimatization rate of 53% was found three months after transfer to <i>ex vitro</i> conditions and the recovered plants presented a normal phenotypic aspect. 收起