摘要 : We identified an inverted, functional cAMP response element (CRE) located at -1599 bp relative to the translation start site within the ovine beta 1-adrenergic receptor (beta 1AR) gene promoter. In transfection studies with SK-N-M... 展开 We identified an inverted, functional cAMP response element (CRE) located at -1599 bp relative to the translation start site within the ovine beta 1-adrenergic receptor (beta 1AR) gene promoter. In transfection studies with SK-N-MC cells, a 40-bp oligonucleotide containing the potential CRE, beta 1AR-CRE, conferred a 3- to 4-fold increase in luciferase activity mediated by cAMP. The induction was mimicked by co-transfecting the cells with a vector overexpressing the alpha-catalytic subunit of the cAMP-dependent protein kinase (PKA) without treatment, and was blocked by overexpressing a PKA inhibitor (PKI). Ln electrophoretic mobility shift assays, a discrete binding pattern was shown in cell nuclear extract probed with the 40 bp beta 1AR-CRE. The binding was shown to be specific and supershifted by addition of a CRE binding protein (CREB-1) antibody. These data demonstrate that cAMP mediates the induction of beta 1AR gene expression by interacting with an inverted CRE within the promoter region. This is the first reported functional CRE among all beta 1AR genes. 收起