摘要
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Nodal and nodal TCLs explants of endangered <i>Ceropegia spiralis</i> and <i>C. pusilla</i> were cultured on Murashige & Skoog media (full, half and quarter strengths) supplied with sucrose, cytokinins and auxins alone and in comb...
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Nodal and nodal TCLs explants of endangered <i>Ceropegia spiralis</i> and <i>C. pusilla</i> were cultured on Murashige & Skoog media (full, half and quarter strengths) supplied with sucrose, cytokinins and auxins alone and in combinations. Flowering was achieved after 5-10 weeks of culture. In <i>C. spiralis</i>, the maximum flowering (84%) was observed on 1/2 MS medium supplemented with NAA 10.74 micro M/l, 3% sucrose and in <i>C. pusilla</i>, the maximum flowering (80%) was recorded on 1/2 MS containing IBA 0.0123 micro M/l, with 3% sucrose. Although these flowers are smaller in size, the <i>in vitro</i> flowers were morphologically comparable with that of <i>in vivo</i> derived flowers.
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