摘要 : <i>Pineapple mealybug wilt associated virus-2</i> (PMWaV-2) is a mealybug transmitted ampelovirus associated with mealybug wilt disease of pineapple (MWP) in Hawaii. The characteristics of PMWaV-2 acquisition and persistence of gr... 展开 <i>Pineapple mealybug wilt associated virus-2</i> (PMWaV-2) is a mealybug transmitted ampelovirus associated with mealybug wilt disease of pineapple (MWP) in Hawaii. The characteristics of PMWaV-2 acquisition and persistence of grey pineapple mealybugs (GPM), <i>Dysmicoccus neobrevipes</i> Beardsley, were measured. Some GPM were able to acquire the virus within 36 hours. Transmission efficiency of GPM in groups of 25 was 100% following a 72-hour acquisition access period (AAP). GPM remained viruliferous for up to three days after a 3-day AAP when serially transferred to virus-free pineapple plants at 24-hour intervals over a 7-day period. Access to a kabocha squash, a non-host of the virus, reduced the transmission efficiency and persistence of the virus. These data show that PMWaV-2 is transmitted by the grey pineapple mealybug in a semi-persistent manner. A potential management strategy based on these findings is discussed. 收起