摘要
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This experiment was conducted to investigate the effect of coconut oil and garlic powder supplementation on digestibility of nutrients, rumen fermentation, rumen ecology, rumen microorganism and methanogen diversity. Four, 3-year ...
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This experiment was conducted to investigate the effect of coconut oil and garlic powder supplementation on digestibility of nutrients, rumen fermentation, rumen ecology, rumen microorganism and methanogen diversity. Four, 3-year old, rumen fistulated swamp buffalo bulls were randomly assigned in a 4x4 Latin square design to receive four dietary treatments; 7% coconut oil, 7% coconut oil with 50 g/day of garlic powder, 7% coconut oil with 100 g/day of garlic powder and non-supplemented (control). During the experiment, concentrate was offered at 0.5% of BW while rice straw was given on <i>ad libitum</i> basis. It was found that supplementation of 7% coconut oil had significantly influenced on total DM intake, OM, NDF and ADF digestibilites while supplementation of 7% coconut oil with garlic powder (50 and 100 g/day) were not significantly different when compared with the control. Dietary supplementations did not affect on rumen pH, NH<sub>3</sub>-N concentrations. Blood urea nitrogen (BUN) was significantly higher in supplemented groups. Total VFA concentration, proportion of acetate and acetate to propionate ratio were reduced by supplementation. Proportion of propionate was increased (<i>P</i><0.05) when supplemented with 7% coconut oil and 7% coconut oil with 100 g/day of garlic powder. Methane production was dramatically reduced (<i>P</i>=0.005) in supplemented treatments and was 10% reduced in 7% coconut oil supplementation. Amylolytic and proteolytic bacteria were increased (<i>P</i>=0.007 and <i>P</i>=0.024) while protozoal population decreased by 68-75% (<i>P</i><0.01) by supplementation. Total bacterial population was increased by supplementation while total fungi and total methanogens were not significantly different among treatments. Percentage of cellulolytic bacterial population was not different among treatments. However, dietary supplementation reduced (<i>P</i><0.001) percentage of <i>F. succinogenes</i> population. However, methanogen diversity was not changed using PCR-DGGE as technique. Thirteen separate bands were found in the DGGE gel. Based on this study, supplementation with 7% coconut oil plus 100 g/day of garlic powder could be efficiently utilized in the rumen and thus, could provide good fermentation end products and improve rumen ecology for the host swamp buffaloes particularly in reducing 9% methane gas production without changing nutrient digestibilities.
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