摘要 : To improve the practical usefulness of the firefly luciferase, we performed gene chimerization between Photinus pyralis luciferase and a thermostable variant of Luciola cruciata luciferase. One chimeric luciferase showed low K_m v... 展开
作者 | Kozo Hirokawa Naoki Kajiyama Seiji Murakami |
---|---|
作者单位 | |
期刊名称 | 《Biochimica et Biophysica Acta.Protein Structure and Molecular Enzymology》 |
总页数 | 9 |
语种/中图分类号 | 英语 / Q5 Q7 |
关键词 | thermostable mutant catalytic efficiency light emission spectrum pH effect |
馆藏号 | N2009EPST0000276 |