摘要 : <i>Broad bean wilt virus</i> 1 (BBWV-1) and BBWV-2 are the two most significant viruses in the genus <i>Fabavirus</i>, causing damage to many economically important agricultural crops worldwide. A quantitative real-time reverse tr... 展开 <i>Broad bean wilt virus</i> 1 (BBWV-1) and BBWV-2 are the two most significant viruses in the genus <i>Fabavirus</i>, causing damage to many economically important agricultural crops worldwide. A quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) procedure using two TaqManReg.MGB probes was developed for sensitive and specific detection and quantitation of BBWV-1 and BBWV-2. Primers and probes were designed from conserved sequence stretches to detect all isolates of each virus. Standard curves using RNA transcripts identical to both TaqManReg.MGB probes enabled absolute quantitation, with a wide dynamic range and high sensitivity (10³-10¹⁰ RNA molecules). RT-qPCR was assayed with genetically divergent BBWV-1 and BBWV-2 isolates from different plant hosts and countries, and was used to evaluate the temporal accumulation of BBWV-1 RNA in two plant hosts. 收起