摘要 : This study was conducted to isolate and characterize the lytic phages from animal waste materials specific against <i>Bacillus subtilis</i> and <i>Escherichia coli</i>. 75 waste water materials consisting of cow, buffalo, goat, pi... 展开 This study was conducted to isolate and characterize the lytic phages from animal waste materials specific against <i>Bacillus subtilis</i> and <i>Escherichia coli</i>. 75 waste water materials consisting of cow, buffalo, goat, pig and poultry excreta were collected from the top, middle and bottom portions of the collection tank. The samples were processed and <i>B. subtilis</i> and <i>E. coli</i> were used as the primary host for isolation, propagation and characterization of phages. Tubidity reduction and phage streaking methods were performed to observed the lytic activity of the bacteria. The lytic activity of the recovered phages were tested against <i>Staphylococcus aureus, Salmonella, Pseudomonas aeruginosa, E. coli, Proteus</i> sp. and <i>Klebsiella</i> sp. isolated from different samples. Results revealed that phages could be collected from wastes of dairy cattle (90%), followed by buffalo (75%), pig (67%), poultry (50%) and ducks (17%). There were 41 phages isolated from 75 samples, wherein 35 and six phages were against <i>B. subtilis</i> and <i>E. coli</i>, respectively. Three different phages were categorized for <i>B. subtilis</i> and <i>E. coli</i> such as BsHR1, BsHR2 and BsHR3, and EHR1, EHR2 and EHR3, respectively. EHR2 had a narrow host range, while EHR2 had a wide host range and showed lytic activity against <i>Salmonella, Pseudomonas aeruginosa, Klebsiella</i> sp. and <i>Proteus</i> sp., except <i>Staphylococcus aureus</i>. BsHR1, BsHR2 and BsHR3 were effective against <i>Pseudomonas aeruginosa</i> and <i>Salmonella</i>. BsHR1, BsHR2 and EHR3 remained viable for 2 min when exposed to heat at 70 degrees C, but were inactivated at 3 min. The phages were completely inactivated at pH 3-11, but remained viable at pH 5-9. 收起