摘要 : The objective of this work is to establish a reliable and reproducible molecular method based on SSRs, which allows us to carry out a molecular characterization of selected <i>Phytophthora cinnamomi</i> resistant trees for their c... 展开 The objective of this work is to establish a reliable and reproducible molecular method based on SSRs, which allows us to carry out a molecular characterization of selected <i>Phytophthora cinnamomi</i> resistant trees for their certification. Molecular analyses were performed on three Asiatic <i>Castanea</i> species used in hybridization programs with <i>C. sativa</i>. Moreover, <i>C. sativa</i> plants including forest provenances, and fruit selections, and four selected <i>P. cinnamomi</i> resistant clones have also been analyzed. Twelve SSRs loci previously described on <i>C. sativa</i> were assayed. The results obtained showed that ten of the twelve SSRs loci tested (KT002a, KT015a, CsCAT1, CsCAT3, CsCAT6, CsCAT14, CsCAT16, EMCs14, QpZag36, QpZag110) generated reproducible fragments and were used for genotype identification. A genetic similarity matrix based of the Dice coefficient was obtained with the microsatellite data. This matrix was used to group all accessions using UPGMA. The generated dendrogram showed two main clusters well defined and separated, with a low genetic similarity coefficient (0.09). Cluster number I was subdivided in two subclusters grouping all <i>C. crenata</i> and <i>C. sativa</i> plants respectively. All selected <i>P. cinnamomi</i> resistant clones grouped with the <i>C. sativa</i> accessions, except one of the selected clones from A Coruna. Cluster number II was also subdivided in two subclusters, grouping all <i>C. mollissima</i> and <i>C. henryi</i> plants respectively. Microsatellite analysis allowed us to assign with some reliability the <i>Phytophthora</i> resistant trees to a given species. Nevertheless, this reliability will be improved by increasing the genetic variability to analyze, which is a work that is currently in progress. 收起