摘要 : The objective of this research was to determine allele and genotype frequencies of the prion protein gene (<i>PRNP</i>) in a flock of sheep and categorise thereof into groups by susceptibility to scrapie. Additionally, a mating si... 展开 The objective of this research was to determine allele and genotype frequencies of the prion protein gene (<i>PRNP</i>) in a flock of sheep and categorise thereof into groups by susceptibility to scrapie. Additionally, a mating simulation was conducted, which aimed at decreasing sheep susceptibility to scrapie. Sheep of Kamieniecka breed from a single flock were investigated. Polymorphism in the PrP gene was identified with the use of the PCR-RFLP method. Four of the five alleles were found - ARR, ARQ, ARH, and VRQ, in nine different combinations - ARR/ARR, ARR/ARQ, ARQ/ARQ, ARR/ARH, ARH/VRQ, ARR/VRQ, ARQ/ARH, ARH/ARH, and ARQ/VRQ. The most frequent allele was the one being the most desired, namely ARR, (63.2%), and the least frequent was VRQ (5.4%). Mating simulation aiming at increasing the frequency of the ARR allele in the examined sheep group demonstrated that in the F6 generation it is possible to obtain as many as 97.7% of homozygotic animals having the lowest susceptibility to scrapie. 收起