摘要 : The use of supercritical fluid (SF) carbon dioxide (CO2) modified by organic solvents and inorganic salts or chelating reagents was investigated for the extraction of the xanthene dyes phloxine B and uranine from soil. Methanol (M... 展开 The use of supercritical fluid (SF) carbon dioxide (CO2) modified by organic solvents and inorganic salts or chelating reagents was investigated for the extraction of the xanthene dyes phloxine B and uranine from soil. Methanol (MeOH), n-butylamine(n-BA), and a chelating agent, ethylenediaminetetraacetic acid tetrasodium salt (Na4EDTA), were the most effective modifiers of SF CO2 for quantitative recoveries of phloxine B and uranine in soils with 10-20% moisture at 60 鳦/476 atm and 60 鳦/272 atm,respectively. At these supercritical fluid extraction (SFE) conditions, recoveries of related xanthene dyes (i.e., 2', 7'-dichlorofluorescein, 4,5,6,7-tetrachlorofluorescein, eosin Y lactone, erythrosin B, and rose bengal) fortified at 25 鎔/g in Hawaiian soils ranged from 65 to 93%. Good separation of a mixture of these dyes was achieved by HPLC. A mixture of MeOH, n-BA, and sodium hexametaphosphate [(NaPO3)6] was effective for conventional solvent extraction of phloxine B and uranine from fortified soils. However, SFE was more selective and gave cleaner extracts. Recoveries were comparable to those by solvent extraction. 收起