摘要 : Reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of viruses from <i>Daphne odora</i> plants in this study. Five sets of primers were designed for the specific detection of five different vir... 展开 Reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of viruses from <i>Daphne odora</i> plants in this study. Five sets of primers were designed for the specific detection of five different viruses, <i>Daphne virus Y</i> (DVY, Potyvirus), <i>Cucumber mosaic virus</i> (CMV, Cucumovirus), <i>Daphne virus S</i> (DVS, Carlavirus), <i>Cycas necrotic stunt virus</i> (CNSV, Nepovirus), and <i>Watermelon mosaic virus</i> (WMV, Potyvirus) in collected <i>Daphne</i> plants. RT-PCR could successfully detect the viruses from the <i>Daphne</i> plants. Two potyviruses (DVY and WMV), CNSV, CMV and DVS were frequently detected in >85% samples examined in this study. CNSV was the most prevalent virus (78%), and the infection rate for the three viruses WMV, CMV and DVS was >60%. These results demonstrate that virus diseases on <i>Daphne</i> plants are extremely severe and widespread in Korea. It is especially notable that mixed virus infections with more than three different viruses were very common and 12% of <i>Daphne</i> plants were infected by the five viruses. The results suggest that five primer sets developed in this study can successfully detect the target viruses and can be useful for the monitoring of virus incidence and indexing of the virus-free <i>Daphne</i> plants. 收起