摘要 : The examination of 300 milk samples by multiplex PCR confirmed 83 strains of <i>S. aureus</i>. All of the tested strains of <i>S. aureus</i> presented haemolysis on blood agar plates: 69 strains (83.13%) showed beta -haemolysis; 1... 展开 The examination of 300 milk samples by multiplex PCR confirmed 83 strains of <i>S. aureus</i>. All of the tested strains of <i>S. aureus</i> presented haemolysis on blood agar plates: 69 strains (83.13%) showed beta -haemolysis; 14 (16.8%) alpha -haemorysis; and none of the isolates produced double haemolysis ( alpha + beta ). All 83 isolates of <i>S. aureus</i> were sensitive to methicillin as detected by the interpretative criteria developed by the NCCLS (2002). For the 83 <i>S. aureus</i> isolates, we compared the methicillin susceptibility results, determined by the standardized agar diffusion assay, with the PCR assay for the detection of the antibiotic resistance <i>mec</i>A gene. For all isolates, we found a correlation between the results of the PCR and those of the classical resistance testing. The results obtained showed, that none of the isolates of <i>S. aureus</i> from the 83 individual milk samples from the experimental dairy farm were positive for the presence of the <i>mec</i>A gene coding for the methicilin resistance. None of the tested strains of <i>S. aureus</i> isolates were positive for the presence of the genes for enterotoxin (<i>sea</i>, <i>seb</i>, <i>sec</i>, <i>sed</i>, <i>see</i>) production. 收起