摘要 : LSD1-related proteins have been found to regulate programmed cell death (PCD) and disease related signaling in the plants. Rice <i>LSD1</i>-like (<i>OsLOL1</i>) gene was involved in regulation of cell death and <i>OsLOL</i><i><sub... 展开 LSD1-related proteins have been found to regulate programmed cell death (PCD) and disease related signaling in the plants. Rice <i>LSD1</i>-like (<i>OsLOL1</i>) gene was involved in regulation of cell death and <i>OsLOL</i><i>₂</i> (rice zinc-finger gene) has been reported to regulate plant growth and disease resistance in plants. Bacterial-wilt is a devastating disease caused by <i>Ralstonia solanacearum</i> that needs to be controlled effectively. To achieve this purpose, Agrobacterium-mediated transgenic tobacco plants were generated, which hold <i>OsLOL</i><i>₂</i> gene in their genome encoding zinc-finger proteins. Selection of the T2 <i>OsLOL2</i> transgenic lines was accomplished using molecular techniques, and later on their physiological characterization was done. Southern-blot assay of the selected transformed lines reveals that the transgene has been incorporated into the tobacco genome. On infection with <i>R. solanacearum</i> strains (GMI1000 and RS1000), the selected T₂ transgenic tobacco lines showed enhanced resistance against bacterial-wilt when compared to control plants. Moreover, relatively less bacterial proliferation occurred in the infected stem-tissues of the transgenic lines. Owing to smaller bacterial populations, relatively fewer blockages in xylem vessels were observed during microscopy in the transgenic lines as compared to control. 收起