摘要
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<i>Lactobacillus acidophilus</i> is one of the most commonly used probiotics. This study aimed to produce low salt (3% Nacl) soft cheese with acceptable organoleptic characters and prolonged shelf-life using <i>Lb. acidophilus</i>...
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<i>Lactobacillus acidophilus</i> is one of the most commonly used probiotics. This study aimed to produce low salt (3% Nacl) soft cheese with acceptable organoleptic characters and prolonged shelf-life using <i>Lb. acidophilus</i> (1 and 3%) as bio preservative. The obtained cheese (control, 1%Lb. and 3%Lb.) were kept in refrigerator, sampled fresh (zero time) and at 3 days intervals till signs of spoilage were detected. Samples were examined organoleptically, chemically and microbiologically. Results showed that 3% Lb. cheese was superior to 1%Lb. and control cheese when fresh with an average organoleptic overall score of 95.96, 94.04 and 90.82, respectively. pH values at zero time for control, 1% Lb. and 3% Lb. cheese were 6.42, 6.33 and 6.17 then decreased at the end of shelf life (at 15th, 18th and 24th day) to 5.89, 5.65 and 5.43, respectively. Average coliforms count (MPN/g) was 3.6x10<sup>1</sup>, 1.1x10<sup>1</sup> and 0.93x10<sup>1</sup> at zero time then reached 7.5x10<sup>4</sup>, 0.93x10<sup>1</sup> and 2.3x10<sup>1</sup> at the end of shelf life for control, 1% Lb. and 3% Lb. cheese, respectively. While, <i>E. coli</i> was absent from all examined low salt soft cheese throughout the entire period. On storage, <i>Lb. acidophilus</i> was-sharply increased in their numbers-1.34x10<sup>8</sup> and 2.18x10<sup>9</sup> cfu/g for 1% Lb. (at the 18th day) and 3% Lb. cheese (at the 24th day). Effect of <i>Lb. acidophilus</i> strain on mould and yeast count were highly significant (P<0.01). In conclusion, low salt soft cheese with 3% <i>Lb. acidophilus</i> culture had better organoleptic score, microbiological quality and prolonged shelf-life than control and 1% Lb cheese.
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