摘要 : f3-aminobutyric acid BABA is a simple, nonprotein amino acid that exhibits postinfectional activity against nematodes, fungi, bacteria and tobacco mosaic virus TMV, but its activity against phytoplasmas has never been examined bef... 展开 f3-aminobutyric acid BABA is a simple, nonprotein amino acid that exhibits postinfectional activity against nematodes, fungi, bacteria and tobacco mosaic virus TMV, but its activity against phytoplasmas has never been examined before. The aim of this research was to examine possible postinfectional activity of BABA against phytoplasma reference strains AY-A and KVI in Cutharanthus roseus shoots grown in vitro Shoots were grown on Murashige and Skoog MS medium supplemented with different concentrations of BABA 10 uM, 100 uM, 1 mM, 10 mM, lOOmM, through five subcultures The length of the shoots infected with phytoplasma strain AY-A was measured at the beginning and at the end of the first three subcultures. Only in the first subculture BABA supported shoot elongation. The presence of phytoplasmas was confirmed by nested PCR in both AY-A and KVI-infected shoots after the first and the fifth subculture, showing that in these experimental conditions BABA had no curative effect against phytoplasmas. 收起