摘要 : In order to develop an alternative method to optimize the relative efficiency factor (REF) assessment, the efficiency of transmission of <i>Potato virus Y</i> (PVY) by seven aphid species was examined. <i>In vitro</i> micropropaga... 展开 In order to develop an alternative method to optimize the relative efficiency factor (REF) assessment, the efficiency of transmission of <i>Potato virus Y</i> (PVY) by seven aphid species was examined. <i>In vitro</i> micropropagated potato plantlets were used to experiment on phenotypically and genetically homogeneous material. Species-specific acquisition access period (AAP) on a PVY-infected plantlet was assessed for each aphid species using electrical penetration graph (EPG) technique. Aphid probing behaviour determined by EPG showed that <i>Macrosiphum euphorbiae</i> and <i>Myzus persicae</i> exhibited the shortest AAPs (15 and 11 min, respectively) whereas <i>Rhopalosiphum padi</i>, <i>Sitobion avenae</i>, <i>Brevicoryne brassicae</i> and <i>Acyrthosiphon pisum</i> exhibited the longest ones (more than 30 min). The transmission rate obtained for <i>M. persicae</i> (83.3%) was higher than the ones reported in the literature. REFs assessment showed that <i>A. pisum</i> and <i>B. brassicae</i> were poor efficient vectors while <i>M. euphorbiae</i> and <i>S. avenae</i> seemed to be efficient ones even though their respective REF were significantly lower than that of <i>M. persicae</i>. The species <i>R. padi</i> and <i>A. fabae</i> did not transmit PVY. The hypothesis assessed for <i>M. euphorbiae</i> and <i>S. avenae</i> and consisting in the compensation of a weak PVY-transmission efficiency by a higher number of vectors, was not supported. The use of this new method for REF evaluation and the need to consider aphid behaviour for such an assessment was discussed. 收起