摘要 : In order to study the placental transfer of nucleoside reverse transcriptase inhibitors, a quick and simple reversed phase high performance liquid chromatography (HPLC) method has been developed and validated using an underivatize... 展开 In order to study the placental transfer of nucleoside reverse transcriptase inhibitors, a quick and simple reversed phase high performance liquid chromatography (HPLC) method has been developed and validated using an underivatized silica column for the separation and analysis of DDC and D4T from rat plasma, amniotic fluid, placental, and fetal homogenate. Extraction of DDC, D4T, and their internal standard lamivudine (3TC) from the matrices was processed by liquid-liquid extraction enhanced by salting out the sample using a saturated solution of ammonium sulfate. Chromatographic separation was achieved on a Waters Spherisorb S3W silica column (4.6mm100mm) equipped with a Phenomenex guard column. The mobile phase consisted of 3% methanol in 22mM formic acid. The flow rate was 0.5mL/min, and the detection wavelength was optimized at 265nm. The calibration curves for each day of validation showed good linear response over the range from 0.1g/mL to 50g/mL. The absolute recoveries for all the drugs are all higher than 75%. All the intra- and inter-day assay precision and accuracy were better than 5% for all the matrices. 收起