摘要 : A biosensor for on-line monitoring of glucose in brain dialysates was developed. The biosensor was generated in the cross-flow amperometric cell by potentiostatic electropolymerization of phenol (50 mM) in the presence of glucose ... 展开 A biosensor for on-line monitoring of glucose in brain dialysates was developed. The biosensor was generated in the cross-flow amperometric cell by potentiostatic electropolymerization of phenol (50 mM) in the presence of glucose oxidase (935 U/mL) at + 600 mV (vs. Ag/AgCl) at a flow-rate of 5 mu L/min at the Pt electrode. The polyphenol film ensured low interferences of ascorbate. The on-line ser-up was mathematically described and the model was used to calculate the extracellular concentration of glucose in the striatum of an anesthetized rat. The concentration of glucose was found to be 1.4 +/- 0.5 mM and 0.9 +/- 0.2 mM in two independent experiments. [References: 27] 收起