摘要 : The effect of plant growth regulators on callus induction using different explants, to regenerate shoot/root from different explants and from calli in cultures of <i>Catharanthus roseus</i> (L.) G. Don was studied. Total of 19 sho... 展开 The effect of plant growth regulators on callus induction using different explants, to regenerate shoot/root from different explants and from calli in cultures of <i>Catharanthus roseus</i> (L.) G. Don was studied. Total of 19 shoot regeneration media were used, out of these three were further used to assess the regeneration response and for root regeneration four media were tested supplemented with different concentration of IBA. Based on the results of this study, for shoot proliferation, MS basal medium supplemented with BAP (1.5 mg/liter)+NAA (1.0 mg/liter) was the best followed by MS medium fortified with BAP (3.0 mg/liter)+NAA (4.0 mg/liter) while half strength MS medium supplemented with IBA (2.5 mg/liter)+NAA (0.5 mg/liter) gave best rooting response with quality roots. Besides, shoot regeneration, response from calli both under light and dark conditions was also studied and the media which gave best results was BAP (1.5 mg/liter)+NAA (1.0 mg/liter). 收起