摘要 : Mitochondria in it variety of cell types respond to physiological Ca2+ oscillations in the cytosol dynamically with Ca2+ uptakes. In heart cells, mitochondrial Ca2+ uptakes occur by a ruthenium red-sensitive Ca2+ uniporter (CaUP),... 展开 Mitochondria in it variety of cell types respond to physiological Ca2+ oscillations in the cytosol dynamically with Ca2+ uptakes. In heart cells, mitochondrial Ca2+ uptakes occur by a ruthenium red-sensitive Ca2+ uniporter (CaUP), a rapid mode of Ca2+ uptake (RaM) and a ryanodine receptor (RyR) localized in the inner mitochondrial membrane (IMM). Three subtypes of RyRs have been described and cloned, however, the subtype identity of the mitochondrial ryanodine receptor (mRyR) is unknown. Using subtype specific antibodies, we characterized the mRyR in the IMM front rat heart as RyR1. These results are substantiated by the absence of RyR protein in heart mitochondria from RyR1 knockout mice. The bell-shape Ca2+-dependent [H-3]ryanodine binding curve and its modulation by caffeine and adenylylmethylenediphosphonate (AMPPCP) give further evidence that mRyR functions pharmacologically like RyR1. Ryanodine prevents mitochondrial Ca2+ uptake induced by raising extramitochondrial Ca2+ to 10 mu M. Similarly, ryanodine inhibits oxidative phosphorylation Stimulated by 10 mu M extramitochondrial Ca2+. In summary, our results show that the mRyR in cardiac muscle has similar biochemical and pharmacological properties to the RyR1 in the sarcoplasmic reticulum (SR) of skeletal muscle. These results could also Suggest an efficient mechanism by which mitochondria sequesters Ca2+ via mRyR during excitation-contraction Coupling to stimulate oxidative phosphorylation for ATP production to meet metabolic demands. Thus, the mRyR functions as a transducer for excitation-metabolism coupling. (c) 2005 Elsevier B.V. All rights reserved. 收起